The presence of dopamine in the lactotroph cell, as well as in isolated prolactin secretory granules, was demonstrated by means of an histochemical reaction for electron microscopy. Biochemical assays further confirmed the presence of dopamine in the secretory granules.
Autoradiographic preparations examined by light microscopy showed dopamine internalization in dispersed anterior pituitary cells.
Isolated anterior pituitary lactotroph cells incorporated more [3H]dopamine than a fraction containing other anterior pituitary cells.
J. Endocr. (1985) 104, 23–28
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