Establishment of a continuous somatostatin-producing line of medullary thyroid carcinoma cells from BALB/c mice

in Journal of Endocrinology
Authors:
A. S. Tischler
Search for other papers by A. S. Tischler in
Current site
Google Scholar
PubMed
Close
,
Y. C. Lee
Search for other papers by Y. C. Lee in
Current site
Google Scholar
PubMed
Close
,
D. Costopoulos
Search for other papers by D. Costopoulos in
Current site
Google Scholar
PubMed
Close
,
G. Nunnemacher
Search for other papers by G. Nunnemacher in
Current site
Google Scholar
PubMed
Close
,
R. A. DeLellis
Search for other papers by R. A. DeLellis in
Current site
Google Scholar
PubMed
Close
,
M. J. Van Zwieten
Search for other papers by M. J. Van Zwieten in
Current site
Google Scholar
PubMed
Close
,
H. J. Wolfe
Search for other papers by H. J. Wolfe in
Current site
Google Scholar
PubMed
Close
, and
S. R. Bloom
Search for other papers by S. R. Bloom in
Current site
Google Scholar
PubMed
Close
Restricted access
Rent on DeepDyve

Sign up for journal news

ABSTRACT

A continuous line of somatostatin-producing medullary thyroid carcinoma cells was established from a transplantable tumour in BALB/c mice. Virtually all of the somatostatin immunoreactivity co-chromatographed with somatostatin 14. The tumour cells replicated in spinner cultures with a doubling time of approximately 4 days, and the concentration of somatostatin released into the culture medium increased in proportion to the number of cells. Two-to threefold increases in amounts of stored and released somatostatin were observed after treatment of the cells with bromodeoxyuridine. This cell line might be valuable for studies of somatostatin regulation in normal and neoplastic C-cells, and for other studies of C-cell biology which require a mouse model.

J. Endocr. (1986) 110, 309–313