Thyrotrophin in individual pituitaries obtained from fetal and prepubertal pigs was quantified by homologous radioimmunoassay (RIA) and heterologous radioreceptor assay (RRA). Relative evolution of pituitary TSH contents and concentrations with age were in good agreement as measured by both assay systems although the quantity of TSH detected by RRA appeared consistently lower than that measured by RIA. Thyrotrophin was first detected in pituitaries of fetal pigs at day 75 of gestation. Thereafter the pituitary content of TSH increased to approximately 45 μg/pituitary in the oldest group tested (6 weeks of age). The pituitary TSH concentration rose sharply until birth (114±1 day post coitum) and thereafter remained increased at a concentration of approximately 400 ng/mg wet weight.
J. Endocr. (1986) 111, 111–115
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