Autoradiographic localization of binding sites for oxytocin and vasopressin in the rat kidney

in Journal of Endocrinology
Restricted access

ABSTRACT

The distribution of [3H]vasopressin- and [3H]oxytocin-binding sites was examined, using an autoradiographical technique, in the kidney of Long–Evans and Brattleboro rats. Two types of binding sites with affinities in the nanomolar range were detected: one, located on glomeruli, bound both vasopressin and oxytocin; the other, on collecting ducts, bound vasopressin selectively. In the presence of 10 μmol oxytocin/1, [3H]vasopressin labelling was abolished in glomeruli, but only reduced in collecting ducts; [3H]oxytocin labelling was completely abolished by 10 μmol vasopressin/1. These observations are discussed in relation to known effects of neurohypophysial hormones on renal physiology.

J. Endocr. (1987) 113, 179–182

 

      Society for Endocrinology

Sept 2018 onwards Past Year Past 30 Days
Abstract Views 120 54 1
Full Text Views 71 1 0
PDF Downloads 103 2 0