Corticotrophin-releasing factor immunoreactivity in the hypothalamus of the rat during the perinatal period

in Journal of Endocrinology
Authors:
A. Chatelain
Search for other papers by A. Chatelain in
Current site
Google Scholar
PubMed
Close
,
F. Boudouresque
Search for other papers by F. Boudouresque in
Current site
Google Scholar
PubMed
Close
,
T. Chautard
Search for other papers by T. Chautard in
Current site
Google Scholar
PubMed
Close
,
J. P. Dupouyt
Search for other papers by J. P. Dupouyt in
Current site
Google Scholar
PubMed
Close
, and
C. Oliver
Search for other papers by C. Oliver in
Current site
Google Scholar
PubMed
Close
Restricted access
Rent on DeepDyve

Sign up for journal news

ABSTRACT

Corticotrophin-releasing factor-41 (CRF-41) immunoreactivity has been measured in hypothalamic extracts of fetal (on days 17, 19 and 21 of gestation), neonatal (1, 2, 3 and 4 weeks of age) and adult rats with a specific radioimmunoassay developed for synthetic rat CRF-41. The hypothalamic content (fmol) and concentration (fmol/mg protein) of immunoreactive CRF-41 gradually increased with age. Chromatography of hypothalamic extracts on Sephadex G-50 Fine showed one single peak of immunoreactive CRF-41 which co-eluted with synthetic rat CRF-41. The retention time of hypothalamic CRF-41 during high-performance liquid chromatography was identical to that of synthetic rat CRF-41 at all stages investigated. These results are consistent with the development of neurones containing CRF-41-like molecules in both the hypothalamus and the median eminence of the fetus, as well as with the hypothalamic control of the cortico-stimulating function of the pituitary gland as early as day 19 of gestation.

J. Endocr. (1988) 119, 59–64

 

  • Collapse
  • Expand