The anti-fertility effect of passive immunization against progesterone is influenced by genotype in mice. In order to quantify this finding we determined the effective dose that blocks pregnancy in 50% of treated mice (ED50) for two different anti-progesterone monoclonal antibodies (DB3 and 11/32) in four different strains of mice (BALB/cJ, CBA/Ca, Tuck's no. 1 and F1C). Efficacy was greatest in the two inbred strains (BALB/cJ and CBA/Ca) with ED50 values of 0·46–1·4 nmol. The F1C hybrid mice were more resistant to antibody treatment (ED50 2·2–3·0 nmol), while the outbred Tuck's no. 1 strain required much higher doses (ED50 7·0–8·2 nmol). There were no intrastrain differences between the two monoclonal antibodies.
We have examined the possible role of the H-2 haplotype on antibody efficacy in different strains and crosses. The antibody was highly effective in blocking implantation in three congenic BALB strains of different H-2 haplotype, in another inbred strain CBA/Ca, and in reciprocal BALB/cJ×CBA/Ca crosses. The F1 hybrid crosses were somewhat resistant, but the C57BL/10Sn and B10.BR congenic strains were most resistant to treatment. The results show that the pregnancy-blocking effect of the anti-progesterone antibody was not influenced by the H-2 haplotype, but rather by background genes.
Journal of Endocrinology (1990) 125, 257–262
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