Stimulation of rat renal medullary Na+/K+-ATPase by arginine vasopressin is mediated by the V2 receptor

in Journal of Endocrinology
Authors:
J. A. Charlton
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P. H. Baylis
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DOI:
https://doi.org/10.1677/joe.0.1270213
Volume/Issue:
Volume 127: Issue 2
Article Type:
Research Article
Page Range:
213–216
Online Publication Date:
Nov 1990
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ABSTRACT

In previous studies, we have demonstrated that 1–10 fmol arginine vasopressin (AVP)/l maximally stimulates the activity of the enzyme Na+/K+-ATPase in the rat renal medullary thick ascending limb (MTAL) of Henle's loop after 4 or 10 min of stimulation when measured using a cytochemical bioassay. We have tested the hypothesis that this stimulation is mediated by the V2 receptor in the MTAL. A cytochemical bioassay was used to investigate the effect of specific V1 and V2/V1 antagonists and a synthetic V2 agonist [1-deamino,8-d-arginine]-vasopressin (dDAVP), on the activity of Na+/K+-ATPase. There was no effect of the V1 antagonist (1 fmol-1 μmol/l) in inhibiting the activity of Na+/K+-ATPase stimulated by 1 fmol AVP/l. In contrast, 100 pmol of the V2/V1 antagonist/l significantly (P < 0·001) inhibited the stimulation of Na+/K+-ATPase activity by 1 fmol AVP/l from 55·5±4·3 (s.e.m.) to 31·9±1·6 mean integrated extinction (MIE) after 4 min of stimulation and from 67·0±3·2 to 36·9±0·7 MIE after 10 min of stimulation. Similarly, the stimulation of Na+/K+-ATPase by 10 fmol dDAVP/l was inhibited by the V2/V1 antagonist from 55·1±1·0 to 26·1±0·5 MIE after 4 min of stimulation.

We conclude that the stimulation of Na+/K+-ATPase by AVP is mediated by the V2 receptor in the rat renal MTAL.

Journal of Endocrinology (1990) 127, 213–216

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Print ISSN:
0022-0795
Online ISSN:
1479-6805
Page(s):
4
Article Type:
Research Article
Print Publication Date:
01 Jan 1990
Online Publication Date:
Nov 1990