Distribution of pyroglutamylpeptide amides related to thyrotrophin-releasing hormone in the central nervous system and periphery of the rat

in Journal of Endocrinology
Authors:
J. del Rio-Garcia
Search for other papers by J. del Rio-Garcia in
Current site
Google Scholar
PubMed Close
and
D. G. Smyth
Search for other papers by D. G. Smyth in
Current site
Google Scholar
PubMed Close
DOI:
https://doi.org/10.1677/joe.0.1270445
Volume/Issue:
Volume 127: Issue 3
Article Type:
Research Article
Page Range:
445–450
Online Publication Date:
Dec 1990
Restricted access
Rent on DeepDyve

Sign up for journal news

ABSTRACT

A rapid and sensitive chromatographic method is presented for determining TRH and TRH-like peptides in small quantities of tissues and fluids. The procedure was applied to screen a comprehensive range of tissues from the central nervous system (CNS) and periphery of the rat. When hydrochloric acid (100 mmol/l) was used for extraction, the TRH immunoreactivity obtained was identified solely with the known tripeptide hormone. In contrast, when weakly acidic conditions were used for extraction, neutral or acidic TRH-like peptides which lacked the histidine residue at position 2 of the hormone, but reacted with TRH antibodies, were shown to be present in addition to TRH. The TRH-like peptides in the brain were located principally in the hippocampus, brain stem and dorsal colliculi. In the periphery, TRH-like peptides were shown to be present in the male reproductive system and certain endocrine tissues. In addition, the presence of TRH-like peptides was confirmed in portal blood. The results indicate that peptides with a neutral or acidic TRH-like sequence at their C-terminus are widely distributed in the CNS and periphery of the rat. These peptides appear to occur mainly or exclusively in precursor forms from which TRH immunoreactivity can be released during extraction under weakly acidic conditions.

Journal of Endocrinology (1990) 127, 445–450

Journal of Endocrinology is committed to supporting researchers in demonstrating the impact of their articles published in the journal.

The two types of article metrics we measure are (i) more traditional full-text views and pdf downloads, and (ii) Altmetric data, which shows the wider impact of articles in a range of non-traditional sources, such as social media.

More information is on the Reasons to publish page.

Sept 2018 onwards Past Year Past 30 Days
Full Text Views 1 0 0
PDF Downloads 1 0 0

 

  • Collapse
  • Expand
Print ISSN:
0022-0795
Online ISSN:
1479-6805
Page(s):
6
Article Type:
Research Article
Print Publication Date:
01 Jan 1990
Online Publication Date:
Dec 1990