Two methods of estimating the individual median effective dose have been studied and found to yield equivalent estimates.
Within-animal variation (over a period of 5 weeks) is shown to be 29% (20–38%) of the between-animal variation. Allen-Doisy assay procedures designed to utilize the withinanimal information therefore require 29% of the observations of the standard method.
The individual median effective dose is a graded character and may be used in the standard procedures of genetical analysis and the analysis of variance.
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