COLOUR CORRECTION IN DETERMINATION OF 17-KETOSTEROIDS BY THE CALLOWZIMMERMANN REACTION

in Journal of Endocrinology
Authors:
ALICE M. ROBINSON
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J. M. CROWLEY
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D. G. JONES
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SUMMARY

The problems of colour correction in the assay of urinary 17-ketosteroids by the Callow-Zimmermann method are critically considered. Details are given of a novel method of deriving correction formulae for two pairs of light filters commonly employed in routine 17-ketosteroid assay. The use of these formulae is illustrated by their application to assays of normal and of pathological urines.

 

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