When hydrocortisone-4-14C is injected intravenously into mice, the whole of the 14C remains in a form which can be recovered from the carcase by extraction with 70% aqueous methanol.
The fraction of recovered 14C which remains soluble in chloroform declines rapidly at first and more slowly thereafter, so that at 1 hr less than 15% is still chloroform soluble.
Glucuronidase increases the chloroform soluble fraction slightly, and a further small amount is made chloroform soluble by acid hydrolysis. The latter fraction seems to increase with time.
The rate of destruction of true hydrocortisone has been measured. There is a rapid decomposition during the first 5 min followed by a slower rate of disappearance during the next hour. The latter corresponds to a rate of metabolism of about 7·8 μg daily. Such a rate is comparable, per gram of tissue, to that believed to occur in man.
The injected 14C is at first excreted mainly into the gut, but later mainly into the urine.
No indication of an altered rate of metabolism of hydrocortisone-4-14C in adrenalectomized mice was obtained.
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