Activation of the hypothalamo-pituitary-adrenocortical axis in the conscious rabbit by the pyrogen polyinosinic: polycytidylic acid is dependent on corticotrophin-releasing factor-41

in Journal of Endocrinology
Authors:
N. G. N. Milton
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E. W. Hillhouse
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A. S. Milton
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DOI:
https://doi.org/10.1677/joe.0.1350069
Volume/Issue:
Volume 135: Issue 1
Article Type:
Research Article
Page Range:
69–75
Online Publication Date:
Oct 1992
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ABSTRACT

The pyrogenic interferon inducer polyinosinic: polycytidylic acid (Poly I: C) was shown to activate the rabbit hypothalamo-pituitary-adrenocortical (HPA) axis in vivo. The immunoreactive cortisol response to Poly I:C (2·5 μg/kg) was shown to have a corticotrophin-releasing factor-41 (CRF-41)-dependent component which was abolished by peripheral immunoneutralization using an anti-CRF41 monoclonal antibody (KCHMB001; 2·5 mg/kg i.v.). Peripheral administration of the arginine vasopressin (AVP) V1 receptor antagonist ([deaminoPen1, O-Me-Tyr2, Arg8]-vasopressin; 225 nmol/kg i.v.) had no effect on the response of immunoreactive cortisol to Poly I:C, suggesting that AVP was not involved in activation of the HPA axis. Poly I: C increased both body temperature and circulating immunoreactive prostaglandin E2; these responses were abolished by the cyclo-oxygenase inhibitor ketoprofen (3 mg/kg s.c.). The immunoreactive cortisol response to Poly I: C, however, remained after the administration of ketoprofen, indicating a prostaglandin (PG)-independent component. The immunoreactive cortisol levels in control, saline vehicle-treated, animals were reduced by both the CRF-41 receptor antagonist (α-helical CRF (9–41); 6·25 mmol/kg i.v.) and ketoprofen (3 mg/kg s.c.) indicating that this basal state is dependent on both CRF-41 and PGs.

Journal of Endocrinology (1992) 135, 69–75

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Print ISSN:
0022-0795
Online ISSN:
1479-6805
Page(s):
7
Article Type:
Research Article
Print Publication Date:
01 Jan 1992
Online Publication Date:
Oct 1992