The ovaries of rats and mice ranging in age from foetal life to sexual maturity have been cultured and observed in vitro. Structures which had some resemblance to oocytes were seen among the epithelial cells which grew out from the ovarian tissue, but it is concluded that they were not oocytes. No evidence for the neoformation of oocytes was obtained.
Serial sections of ovaries, which had been fixed after various periods of culture, were examined for signs of oogenesis. Numerous oocytes in the prophase of cell division were seen in foetal and neonatal material, but not in cultures of adult, or even prepubertal material.
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