Dexamethasone potentiates the stimulatory effect of insulin-like growth factor-I on collagen production in cultured human fibroblasts

in Journal of Endocrinology
Authors:
J L E Bird
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J A Tyler
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DOI:
https://doi.org/10.1677/joe.0.1420571
Volume/Issue:
Volume 142: Issue 3
Article Type:
Research Article
Page Range:
571–579
Online Publication Date:
Sep 1994
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Abstract

We examined the effects of insulin-like growth factor-I (IGF-I) and dexamethasone on the production of collagen by cultures of human infant foreskin fibroblasts, and the interaction between these two factors. IGF-I at 500 ng/ml maximally increased collagen accumulation fourfold. Collagen was increased twofold relative to total protein production. Dexamethasone at a concentration of 1 μmol/l reduced collagen production by between 25% and 40% in unstimulated cells and those cultured with up to 100 ng IGF-I/ml. However, dexamethasone did potentiate collagen production in cells stimulated with 250 ng IGF-I/ml. This potentiation was independent of any effects of IGF-I or dexamethasone on prostaglandin (PG)E2 production. Transforming growth factor-β (TGF-β) is also a potent stimulator of collagen formation. However, no potentiation of TGF-β-stimulated collagen production by dexamethasone was apparent. The mechanism by which dexamethasone potentiates IGF-I-stimulated collagen production was investigated. Dexamethasone treatment increased IGF-I binding to the type 1 IGF receptor without altering the binding affinity. Dexamethasone also attenuated the secretion of IGF-binding proteins by IGF-I-maintained cells.

Journal of Endocrinology (1994) 142, 571–579

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Print ISSN:
0022-0795
Online ISSN:
1479-6805
Page(s):
9
Article Type:
Research Article
Print Publication Date:
01 Jan 1994
Online Publication Date:
Sep 1994