Concentration of free growth hormone-binding protein in the serum of mice is not regulated by growth hormone

in Journal of Endocrinology
Authors:
A I Sotelo
Search for other papers by A I Sotelo in
Current site
Google Scholar
PubMed Close
,
F P Dominici
Search for other papers by F P Dominici in
Current site
Google Scholar
PubMed Close
,
A Bartke
Search for other papers by A Bartke in
Current site
Google Scholar
PubMed Close
, and
D Turyn
Search for other papers by D Turyn in
Current site
Google Scholar
PubMed Close
DOI:
https://doi.org/10.1677/joe.0.1530319
Volume/Issue:
Volume 153: Issue 2
Article Type:
Research Article
Page Range:
319–325
Online Publication Date:
May 1997
Restricted access
Rent on DeepDyve

Sign up for journal news

Abstract

Ames dwarf mice that do not express growth hormone (GH) or prolactin (PRL) genes were used to study the effects of GH deficiency on the presence and the characteristics of GH-binding protein (GHBP) in serum. Chromatographic techniques were used to allow characterization of biological rather than immunological activity of GHBP. Two GH-binding fractions were found in dwarf mice serum, one with low affinity and high capacity (GHBPI) and one with high affinity, low capacity and lower molecular mass (GHBPII). Serum concentration of the high-affinity GHBP was 0·73 ± 0·03 nm with a K d of 6·3 ± 1·7 nm. Since Ames dwarf mice have no GH in the circulation, all the GHBP is free. Interestingly, the concentration of GHBP in dwarf mice was similar to the levels of free GHBP measured in normal mice from the same line. Moreover, this value (0·7 nm) closely resembles the concentration of free GHBP in the serum of transgenic mice overexpressing GH, in which peripheral GH levels are grossly elevated. These observations can be interpreted as evidence that the levels of free GHBP in mouse serum are independent of GH concentration, and that GH influences only the levels of bound GHBP in peripheral circulation.

Journal of Endocrinology (1997) 153, 319–325

Journal of Endocrinology is committed to supporting researchers in demonstrating the impact of their articles published in the journal.

The two types of article metrics we measure are (i) more traditional full-text views and pdf downloads, and (ii) Altmetric data, which shows the wider impact of articles in a range of non-traditional sources, such as social media.

More information is on the Reasons to publish page.

Sept 2018 onwards Past Year Past 30 Days
Full Text Views 3 1 0
PDF Downloads 2 0 0

 

  • Collapse
  • Expand
Print ISSN:
0022-0795
Online ISSN:
1479-6805
Page(s):
7
Article Type:
Research Article
Print Publication Date:
01 Jan 1997
Online Publication Date:
May 1997