Ram spermatozoa were found at the ovarian end of the uterine (Fallopian) tubes of ewes at about 3 hr after mating. No radiopaque medium passed from the vagina to the uterine horns of rabbits following vulval stimulation and copulation. Ram spermatozoa entered the tubes of ewes through a valve-like utero-tubal junction which prevented fluid from passing in the opposite direction. Almost all ram spermatozoa became immotile within 8 hr of their introduction into the tubes of ewes. The method of sperm transport in the female genital tract is discussed.
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