Pooled urine from normal men, normally menstruating women and hospitalized postmenopausal subjects was extracted by six published methods and assayed for human pituitary gonadotrophic (HPG) activity by the mouse uterus test in terms of HMG-20A.
In the three types of urine studied comparable yields of HPG were obtained by all six extraction methods, and an analysis of variance showed the absence of significant differences between the methods.
No evidence could be obtained to support previous claims that the kaolinacetone method of Loraine & Brown (1959) gave less satisfactory yields of urinary HPG than certain of the other procedures.
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