Histological examination (azan method) of the islets of Langerhans of the chick embryo showed that β cells were present on the 11–12th day of incubation, but it was difficult to be certain that granules were present in the cells on those days. An occasional granulated β cell could be seen on the 12th day but granulation became general by the 16th day.
Histochemical tests (by the Barrnett & Seligman technique) indicated the presence of protein-bound sulphydryl groups in the β cells from the 14th day of incubation onwards.
Bioassays of the pancreas by the rat diaphragm method showed that insulin was, in fact, present from the 12th day of incubation onwards.
There is no evidence for the presence of insulin in egg yolk, since yolk extracts did not enhance the glucose uptake of rat diaphragm.
Alloxan did not inhibit the development of β cells in the pancreas of chick embryos.
Journal of Endocrinology is committed to supporting researchers in demonstrating the impact of their articles published in the journal.
The two types of article metrics we measure are (i) more traditional full-text views and pdf downloads, and (ii) Altmetric data, which shows the wider impact of articles in a range of non-traditional sources, such as social media.
More information is on the Reasons to publish page.
| Sept 2018 onwards | Past Year | Past 30 Days | |
|---|---|---|---|
| Full Text Views | 10 | 0 | 0 |
| PDF Downloads | 3 | 0 | 0 |
Online ISSN: 1479-6805
Print ISSN: 0022-0795
Strengthening biomedical communities
to advance science and health
CONTACT US
Bioscientifica Ltd | Starling House | 1600 Bristol Parkway North | Bristol BS34 8YU | UK
Bioscientifica Ltd | Registered in England no 3190519
