An electrophoretically homogeneous component obtained by starch gel electrophoresis of sheep growth hormone (GH) has been used to prepare antiserum in rabbits. By means of a haemagglutination—inhibition reaction, this antiserum was used to assay GH both in sheep pituitary extracts and in sheep sera. The values for the GH content of a number of pituitary extracts obtained by both immunological and biological assay methods were in good agreement. GH levels in sheep serum were found to range between 38 and 600 μg./100 ml.
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