Myo-inositol alters 13C-labeled fatty acid metabolism in human placental explants

in Journal of Endocrinology
Authors:
Oliver C Watkins Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore

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Mohammed Omedul Islam Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore

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Preben Selvam Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore

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Reshma Appukuttan Pillai Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore

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Amaury Cazenave-Gassiot Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore
Singapore Lipidomics Incubator, Life Sciences Institute, National University of Singapore, Singapore, Singapore

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Anne K Bendt Singapore Lipidomics Incubator, Life Sciences Institute, National University of Singapore, Singapore, Singapore

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Neerja Karnani Singapore Institute for Clinical Sciences, Agency for Science, Technology and Research, Singapore, Singapore

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Keith M Godfrey MRC Lifecourse Epidemiology Unit and NIHR Southampton Biomedical Research Centre, University of Southampton and University Hospital Southampton NHS Foundation Trust, Southampton, UK

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Rohan M Lewis MRC Lifecourse Epidemiology Unit and NIHR Southampton Biomedical Research Centre, University of Southampton and University Hospital Southampton NHS Foundation Trust, Southampton, UK

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Markus R Wenk Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore
Singapore Lipidomics Incubator, Life Sciences Institute, National University of Singapore, Singapore, Singapore

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Shiao-Yng Chan Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore
Singapore Institute for Clinical Sciences, Agency for Science, Technology and Research, Singapore, Singapore

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Correspondence should be addressed to S-Y Chan: obgchan@nus.edu.sg
DOI:
https://doi.org/10.1530/JOE-19-0267
Volume/Issue:
Volume 243: Issue 1
Article Type:
Research Article
Page Range:
73–84
Online Publication Date:
Oct 2019
Copyright:
© 2019 Society for Endocrinology 2019
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We postulate that myo-inositol, a proposed intervention for gestational diabetes, affects transplacental lipid supply to the fetus. We investigated the effect of myo-inositol on fatty acid processing in human placental explants from uncomplicated pregnancies. Explants were incubated with 13C-labeled palmitic acid, 13C-oleic acid and 13C-docosahexaenoic acid across a range of myo-inositol concentrations for 24 h and 48 h. The incorporation of labeled fatty acids into individual lipids was quantified by liquid chromatography mass spectrometry. At 24 h, myo-inositol increased the amount of 13C-palmitic acid and 13C-oleic-acid labeled lipids (median fold change relative to control = 1). Significant effects were seen with 30 µM myo-inositol (physiological) for 13C-palmitic acid-lysophosphatidylcholines (1.26) and 13C-palmitic acid-phosphatidylethanolamines (1.17). At 48 h, myo-inositol addition increased 13C-oleic-acid-lipids but decreased 13C-palmitic acid and 13C-docosahexaenoic-acid lipids. Significant effects were seen with 30 µM myo-inositol for 13C-oleic-acid-phosphatidylcholines (1.25), 13C-oleic-acid-phosphatidylethanolamines (1.37) and 13C-oleic-acid-triacylglycerols (1.32) and with 100 µM myo-inositol for 13C-docosahexaenoic-acid-triacylglycerols (0.78). Lipids labeled with the same 13C-fatty acid showed similar responses when tested at the same time point, suggesting myo-inositol alters upstream processes such as fatty acid uptake or activation. Myo-inositol supplementation may alter placental lipid physiology with unknown clinical consequences.

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Print ISSN:
0022-0795
Online ISSN:
1479-6805
Copyright:
© 2019 Society for Endocrinology 2019
Page(s):
12
Article Type:
Research Article
Received Date:
19 Jul 2019
Accepted Date:
12 Aug 2019
Print Publication Date:
01 Oct 2019
Online Publication Date:
Oct 2019
Keywords:
placenta; pregnancy; fatty acids; phospholipids; metabolism

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