Adhesion G-protein-coupled receptor, GPR56, is required for Müllerian duct development in the chick

in Journal of Endocrinology
Authors:
Zahida Yesmin Roly Department of Anatomy and Developmental Biology, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia

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Andrew T Major Department of Anatomy and Developmental Biology, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia

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Alex Fulcher Monash Micro Imaging, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia

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Martin A Estermann Department of Anatomy and Developmental Biology, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia

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Claire E Hirst Department of Anatomy and Developmental Biology, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia

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Craig A Smith Department of Anatomy and Developmental Biology, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia

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Correspondence should be addressed to C A Smith: craig.smith@monash.edu
DOI:
https://doi.org/10.1530/JOE-19-0419
Volume/Issue:
Volume 244: Issue 2
Article Type:
Research Article
Page Range:
395–413
Online Publication Date:
Feb 2020
Copyright:
© 2020 Society for Endocrinology 2020
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The embryonic Müllerian ducts give rise to the female reproductive tract (fallopian tubes, uterus and upper vagina in humans, the oviducts in birds). Embryonic Müllerian ducts initially develop in both sexes, but later regress in males under the influence of anti-Müllerian hormone. While the molecular and endocrine control of duct regression in males have been well studied, early development of the ducts in both sexes is less well understood. Here, we describe a novel role for the adhesion G protein-coupled receptor, GPR56, in development of the Müllerian ducts in the chicken embryo. GPR56 is expressed in the ducts of both sexes from early stages. The mRNA is present during the elongation phase of duct formation, and it is restricted to the inner Müllerian duct epithelium. The putative ligand, Collagen III, is abundantly expressed in the Müllerian duct at the same developmental stages. Knockdown of GPR56 expression using in ovo electroporation results in variably truncated ducts, with a loss of expression of both epithelial and mesenchymal markers of duct development. Over-expression of GPR56 in vitro results in enhanced cell proliferation and cell migration. These results show that GPR56 plays an essential role in avian Müllerian duct development through the regulation of duct elongation.

Supplementary Materials

    • Suppl. Fig 1: Over-expression of GPR56in Müllerian duct has no effect on morphology E6.5 (HH30) chicken urogenital system
    • Supplementary Video 1
    • Supplementary Video 2

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Print ISSN:
0022-0795
Online ISSN:
1479-6805
Copyright:
© 2020 Society for Endocrinology 2020
Page(s):
19
Article Type:
Research Article
Received Date:
05 Dec 2019
Accepted Date:
09 Dec 2019
Print Publication Date:
01 Feb 2020
Online Publication Date:
Feb 2020
Keywords:
female reproduction; Müllerian duct; GPR56; chicken embryo