CaV1.2 and CaV1.3 voltage-gated L-type Ca2+ channels in rat white fat adipocytes

in Journal of Endocrinology
Authors:
Olena A Fedorenko School of Life Sciences, University of Nottingham, Nottingham, UK

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Pawitra Pulbutr Faculty of Pharmacy, Mahasarakham University, Mahasarakham, Thailand

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Elin Banke Department of Physiology/Metabolic Physiology, Institute of Neuroscience and Physiology, The Sahlgrenska Academy at University of Gothenburg, Göteborg, Sweden

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Nneoma E Akaniro-Ejim School of Life Sciences, University of Nottingham, Nottingham, UK

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Donna C Bentley School of Sport, Exercise and Health Sciences, Loughborough University, Loughborough, UK

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Charlotta S Olofsson Department of Physiology/Metabolic Physiology, Institute of Neuroscience and Physiology, The Sahlgrenska Academy at University of Gothenburg, Göteborg, Sweden

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Sue Chan School of Life Sciences, University of Nottingham, Nottingham, UK

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Paul A Smith School of Life Sciences, University of Nottingham, Nottingham, UK

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Correspondence should be addressed to P A Smith: Paul.a.smith@nottingham.ac.uk
DOI:
https://doi.org/10.1530/JOE-19-0493
Volume/Issue:
Volume 244: Issue 2
Article Type:
Research Article
Page Range:
369–381
Online Publication Date:
Feb 2020
Copyright:
© 2020 Society for Endocrinology 2020
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L-type channel antagonists are of therapeutic benefit in the treatment of hyperlipidaemia and insulin resistance. Our aim was to identify L-type voltage-gated Ca2+ channels in white fat adipocytes, and determine if they affect intracellular Ca2+, lipolysis and lipogenesis. We used a multidisciplinary approach of molecular biology, confocal microscopy, Ca2+ imaging and metabolic assays to explore this problem using adipocytes isolated from adult rat epididymal fat pads. CaV1.2, CaV1.3 and CaV1.1 alpha1, beta and alpha2delta subunits were detected at the gene expression level. The CaV1.2 and CaV1.3 alpha1 subunits were identified in the plasma membrane at the protein level. Confocal microscopy with fluorescent antibodies labelled CaV1.2 in the plasma membrane. Ca2+ imaging revealed that the intracellular Ca2+ concentration, [Ca2 +]i was reversibly decreased by removal of extracellular Ca2+, an effect mimicked by verapamil, nifedipine and Co2+, all blockers of L-type channels, whereas the Ca2+ channel agonist BAY-K8644 increased [Ca2+]i. The finding that the magnitude of these effects correlated with basal [Ca2+]i suggests that adipocyte [Ca2+]i is controlled by L-type Ca2+ channels that are constitutively active at the adipocyte depolarized membrane potential. Pharmacological manipulation of L-type channel activity modulated both basal and catecholamine-stimulated lipolysis but not insulin-induced glucose uptake or lipogenesis. We conclude that white adipocytes have constitutively active L-type Ca2+ channels which explains their sensitivity of lipolysis to Ca2+ channel modulators. Our data suggest CaV1.2 as a potential novel therapeutic target in the treatment of obesity.

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Print ISSN:
0022-0795
Online ISSN:
1479-6805
Copyright:
© 2020 Society for Endocrinology 2020
Page(s):
13
Article Type:
Research Article
Received Date:
25 Oct 2019
Accepted Date:
21 Nov 2019
Print Publication Date:
01 Feb 2020
Online Publication Date:
Feb 2020
Keywords:
adipocyte; calcium channel; lipolysis; obesity

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