miR-7 mediates the signaling pathway of NE affecting FSH and LH synthesis in pig pituitary

in Journal of Endocrinology
Authors:
Xin Li State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, People’s Republic of China

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Hongjiao Li State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, People’s Republic of China
College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, People’s Republic of China

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Di Zhang College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, People’s Republic of China

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Guojin Xu State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, People’s Republic of China

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Jinglin Zhang Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, People’s Republic of China

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Sheng Cui State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, People’s Republic of China
College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, People’s Republic of China
Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, People’s Republic of China
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, People’s Republic of China

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Correspondence should be addressed to J Zhang or S Cui: 2253055127@qq.com or cuisheng@cau.edu.cn

*(X Li and H Li contributed equally to this work)

DOI:
https://doi.org/10.1530/JOE-19-0331
Volume/Issue:
Volume 244: Issue 3
Article Type:
Research Article
Page Range:
459–471
Online Publication Date:
Mar 2020
Copyright:
© 2020 Society for Endocrinology 2020
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MicroRNA-7 (miR-7) is an important modulator of a plenty of gene expressions and the interrelated biological processes, highly expressed in porcine pituitary. Norepinephrine (NE), acting as an important neurotransmitter or/and a hormone secreted excessively under stress, affects the synthesis and secretion of various hormones, including pituitary follicle-stimulating hormone (FSH) and luteinizing hormone (LH), which are the key hormones which regulate sexual maturation and reproductive functions. However, the relationship among NE, miR-7 and gonadotropin needs to be elucidated. The aim of this study was to identify whether miR-7 involved in the NE-adrenoceptor signaling pathway affects the synthesis and secretion of FSH and LH in porcine pituitary. Our results showed that the NE intracerebroventricular injection increased pituitary miR-7 level and the synthesis and secretion of FSH and LH in porcine, whereas the inhibition of either endogenous miR-7 or β-adrenergic receptors hindered the rise of FSH and LH synthesis induced by NE in cultured primary porcine anterior pituitary cells. Further, we identified the molecular type of β-adrenergic receptors and the signaling pathway in porcine pituitary, and we found that NE played its roles relying on adrenoceptor beta 2 (β2AR) and the RAF/MEK/ERK1/2 signaling pathway. The phosphorylation of ERK1/2 upregulated miR-7 level which subsequently enhanced FSH and LH synthesis by targeting to Golgi glycoprotein 1 (GLG1). These suggest that miR-7 mediates NE’s effect on promoting FSH and LH synthesis in porcine pituitary.

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Print ISSN:
0022-0795
Online ISSN:
1479-6805
Copyright:
© 2020 Society for Endocrinology 2020
Page(s):
13
Article Type:
Research Article
Received Date:
21 Dec 2019
Accepted Date:
06 Jan 2020
Print Publication Date:
01 Mar 2020
Online Publication Date:
Mar 2020
Keywords:
miR-7; norepinephrine; gonadotropin; pituitary; pig

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