Betatrophin knockdown induces beiging and mitochondria biogenesis of white adipocytes

in Journal of Endocrinology
Authors:
Zhe-Zhen Liao Department of Metabolism and Endocrinology, The First Affiliated Hospital of University of South China, Hengyang, Hunan Province, China

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Xiao-Yan Qi Department of Metabolism and Endocrinology, The First Affiliated Hospital of University of South China, Hengyang, Hunan Province, China

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Ya-Di Wang Department of Metabolism and Endocrinology, The First Affiliated Hospital of University of South China, Hengyang, Hunan Province, China

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Jiao-Yang Li Department of Metabolism and Endocrinology, The First Affiliated Hospital of University of South China, Hengyang, Hunan Province, China

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Qian-Qian Gu Department of Metabolism and Endocrinology, The First Affiliated Hospital of University of South China, Hengyang, Hunan Province, China

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Can Hu Department of Metabolism and Endocrinology, The First Affiliated Hospital of University of South China, Hengyang, Hunan Province, China

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Yin Hu Department of Metabolism and Endocrinology, The First Affiliated Hospital of University of South China, Hengyang, Hunan Province, China

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Heng Sun Department of Metabolism and Endocrinology, The First Affiliated Hospital of University of South China, Hengyang, Hunan Province, China

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Li Ran Department of Metabolism and Endocrinology, The First Affiliated Hospital of University of South China, Hengyang, Hunan Province, China

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Jing Yang Department of Metabolism and Endocrinology, The First Affiliated Hospital of University of South China, Hengyang, Hunan Province, China

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Jiang-Hua Liu Department of Metabolism and Endocrinology, The First Affiliated Hospital of University of South China, Hengyang, Hunan Province, China

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Xin-Hua Xiao Department of Metabolism and Endocrinology, The First Affiliated Hospital of University of South China, Hengyang, Hunan Province, China

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Correspondence should be addressed to X-H Xiao: xinhua0102@163.com

*(Z-Z Liao and X-Y Qi contributed equally to this work)

DOI:
https://doi.org/10.1530/JOE-19-0447
Volume/Issue:
Volume 245: Issue 1
Article Type:
Research Article
Page Range:
93–100
Online Publication Date:
Apr 2020
Copyright:
© 2020 Society for Endocrinology 2020
Restricted access
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Remodeling of energy-storing white fat into energy-consuming beige fat has led to a promising new approach to alleviate adiposity. Several studies have shown adipokines can induce white adipose tissue (WAT) beiging through autocrine or paracrine actions. Betatrophin, a novel adipokine, has been linked to energy expenditure and lipolysis but not clearly clarified. Here, we using high-fat diet-induced obesity to determine how betatrophin modulate beiging and adiposity. We found that betatrophin-knockdown mice displayed less white fat mass and decreased plasma TG and NEFA levels. Consistently, inhibition of betatrophin leads to the phenotype change of adipocytes characterized by increased mitochondria contents, beige adipocytes and mitochondria biogenesis-specific markers both in vivo and in vitro. Of note, blocking AMP-activated protein kinase (AMPK) signaling pathway is able to abolish enhanced beige-like characteristics in betatrophin-knockdown adipocytes. Collectively, downregulation of betatrophin induces beiging in white adipocytes through activation of AMPK signaling pathway. These processes suggest betatrophin as a latent therapeutic target for obesity.

Supplementary Materials

    • supplementary Figure 1
    • supplementary Figure 2
    • Supplementary table.1 Primary antibodies used for western blotting
    • Supplementary Table 2. Primer sequences used for quantitative RT-PCR

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Print ISSN:
0022-0795
Online ISSN:
1479-6805
Copyright:
© 2020 Society for Endocrinology 2020
Page(s):
8
Article Type:
Research Article
Received Date:
21 Jan 2020
Accepted Date:
06 Feb 2020
Print Publication Date:
01 Apr 2020
Online Publication Date:
Apr 2020
Keywords:
betatrophin; obesity; beiging; mitochondria biogenesis