Altered pancreas remodeling following glucose intolerance in pregnancy in mice

in Journal of Endocrinology
Authors:
Sandra K Szlapinski Department of Physiology and Pharmacology, Western University, London, Ontario, Canada
Lawson Health Research Institute, St Joseph’s Health Care, London, Ontario, Canada

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Anthony A Botros Department of Physiology and Pharmacology, Western University, London, Ontario, Canada
Lawson Health Research Institute, St Joseph’s Health Care, London, Ontario, Canada

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Sarah Donegan Department of Physiology and Pharmacology, Western University, London, Ontario, Canada
Lawson Health Research Institute, St Joseph’s Health Care, London, Ontario, Canada

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Renee T King Lawson Health Research Institute, St Joseph’s Health Care, London, Ontario, Canada

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Gabrielle Retta Lawson Health Research Institute, St Joseph’s Health Care, London, Ontario, Canada

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Brenda J Strutt Department of Physiology and Pharmacology, Western University, London, Ontario, Canada
Lawson Health Research Institute, St Joseph’s Health Care, London, Ontario, Canada

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David J Hill Department of Physiology and Pharmacology, Western University, London, Ontario, Canada
Lawson Health Research Institute, St Joseph’s Health Care, London, Ontario, Canada

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Correspondence should be addressed to S K Szlapinski: sszlapin@uwo.ca
DOI:
https://doi.org/10.1530/JOE-20-0012
Volume/Issue:
Volume 245: Issue 2
Article Type:
Research Article
Page Range:
315–326
Online Publication Date:
May 2020
Copyright:
© 2020 Society for Endocrinology 2020
Restricted access
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Gestational diabetes mellitus increases the risk of dysglycemia postpartum, in part, due to pancreatic β-cell dysfunction. However, no histological evidence exists comparing endocrine pancreas after healthy and glucose-intolerant pregnancies. This study sought to address this knowledge gap, in addition to exploring the contribution of an inflammatory environment to changes in endocrine pancreas after parturition. We used a previously established mouse model of gestational glucose intolerance induced by dietary low protein insult from conception until weaning. Pancreas and adipose samples were collected at 7, 30 and 90 days postpartum for histomorphometric and cytokine analyses, respectively. Glucose tolerance tests were performed prior to euthanasia and blood was collected via cardiac puncture. Pregnant female mice born to dams fed a low protein diet previously shown to develop glucose intolerance at late gestation relative to controls continued to be glucose intolerant until 1 month postpartum. However, glucose tolerance normalized by 3 months postpartum. Glucose intolerance at 7 days postpartum was associated with lower beta- and alpha-cell fractional areas and higher adipose levels of pro-inflammatory cytokine, interleukin-6. By 3 months postpartum, a compensatory increase in the number of small islets and a higher insulin to glucagon ratio likely enabled euglycemia to be attained in the previously glucose-intolerant mice. The results show that impairments in endocrine pancreas compensation in hyperglycemic pregnancy persist after parturition and contribute to prolonged glucose intolerance. These impairments may increase the susceptibility to development of future type 2 diabetes.

Supplementary Materials

    • Supplemental Figure 1. A) Fasting blood glucose levels did not vary after parturition between dietary groups. Values represented are mean ± SEM analyzed by two-way ANOVA, P>0.05. B) Area under the glucose tolerance curve was higher in LPP animals at PPD7 compared to controls. Values represented are mean ± SEM analyzed by unpaired two-tailed Student’s t-test, ** P<0.01. C) There were no differences in proportion of beta-cell proliferation (visualized by cell counting of dual-stained insulin and proliferation marker, ki67, positive cells) found relative to all counted beta cells. Values represented are mean ± SEM analyzed by two-way ANOVA, P>0.05.

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Print ISSN:
0022-0795
Online ISSN:
1479-6805
Copyright:
© 2020 Society for Endocrinology 2020
Page(s):
12
Article Type:
Research Article
Received Date:
20 Feb 2020
Accepted Date:
13 Mar 2020
Print Publication Date:
01 May 2020
Online Publication Date:
May 2020
Keywords:
beta-cell; alpha-cell; postpartum; gestational diabetes mellitus; inflammation

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