SCFAs alleviated steatosis and inflammation in mice with NASH induced by MCD

in Journal of Endocrinology
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  • 1 Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China
  • 2 Key Laboratory of Precision Nutrition and Food Quality, Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China
  • 3 Beijing Laboratory for Food Quality and Safety, China Agricultural University, Beijing, China
  • 4 Research Center for Probiotics, China Agricultural University, Beijing, China
  • 5 School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing, China
  • 6 Hebei Engineering Research Center of Animal Product, Sanhe, China
  • 7 Department of Nutrition, Texas A&M University, College Station, Texas, USA

Correspondence should be addressed to C Wu or L Zhao: cdwu@tamu.edu or lzhao@cau.edu.cn
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This study aimed to assess the effects of three major SCFAs (acetate, propionate, and butyrate) on NASH phenotype in mice. C57BL/6 mice were fed a methionine- and choline-deficient (MCD) diet and treated with sodium acetate, sodium propionate, or sodium butyrate during the 6-week feeding period. SCFA treatment significantly reduced serum levels of alanine aminotransferase and aspartate transaminase, the numbers of lipid droplets, and the levels of triglycerides and cholesterols in livers of the mice compared with control treatment. SCFAs also reduced MCD-induced hepatic aggregation of macrophages and proinflammatory responses. Among the three SCFAs, sodium acetate (NaA) revealed the best efficacy at alleviating MCD-induced hepatic steatosis and inflammation. Additionally, NaA increased AMP-activated protein kinase activation in the liver and induced the expression of fatty acid oxidation gene in both the liver and cultured hepatocytes. In vitro, NaA decreased MCD-mimicking media-induced proinflammatory responses in macrophages to a greater extent than in hepatocytes. These results indicated that NaA alleviates steatosis in a manner involving AMPK activation. Also, NaA alleviation of hepatic inflammation appears to be due to, in large part, suppression of macrophage proinflammatory activation. SCFAs may represent as a novel and viable approach for alleviating NASH.

 

      Society for Endocrinology

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