miR-188 promotes liver steatosis and insulin resistance via the autophagy pathway

in Journal of Endocrinology
Authors:
Ya Liu Department of Endocrinology, Endocrinology Research Center, Xiangya Hospital of Central South University, Changsha, Hunan, China

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Xiaoqing Zhou Department of Endocrinology, Endocrinology Research Center, Xiangya Hospital of Central South University, Changsha, Hunan, China

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Ye Xiao Department of Endocrinology, Endocrinology Research Center, Xiangya Hospital of Central South University, Changsha, Hunan, China

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Changjun Li Department of Endocrinology, Endocrinology Research Center, Xiangya Hospital of Central South University, Changsha, Hunan, China

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Yan Huang Department of Endocrinology, Endocrinology Research Center, Xiangya Hospital of Central South University, Changsha, Hunan, China

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Qi Guo Department of Endocrinology, Endocrinology Research Center, Xiangya Hospital of Central South University, Changsha, Hunan, China

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Tian Su Department of Endocrinology, Endocrinology Research Center, Xiangya Hospital of Central South University, Changsha, Hunan, China

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Lei Fu Department of Infectious Diseases, Xiangya Hospital of Central South University, Changsha, Hunan, China

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Liping Luo Department of Endocrinology, Endocrinology Research Center, Xiangya Hospital of Central South University, Changsha, Hunan, China

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Correspondence should be addressed to L Fu and L Luo: fulei92@126.com or lipingluo1987@gmail.com

*(Y Liu and X Zhou contributed equally to this work)

DOI:
https://doi.org/10.1530/JOE-20-0033
Volume/Issue:
Volume 245: Issue 3
Article Type:
Research Article
Page Range:
411–423
Online Publication Date:
Jun 2020
Copyright:
© 2020 Society for Endocrinology 2020
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Nonalcoholic fatty liver disease (NAFLD) is becoming the most prevalent liver disease worldwide, is characterized by liver steatosis and is often accompanied with other pathological features such as insulin resistance. However, the underlying mechanisms are not fully understood, and specific pharmacological agents need to be developed. Here, we investigated the role of microRNA-188 (miR-188) as a negative regulator in hepatic glucose and lipid metabolism. miR-188 was upregulated in the liver of obese mice. Loss of miR-188 alleviated diet-induced hepatosteatosis and insulin resistance. In contrast, liver-specific overexpression of miR-188 aggravated hepatic steatosis and insulin resistance during high-fat diet feeding. Mechanistically, we found that the negative effects of miR-188 on lipid and glucose metabolism were mediated by the autophagy pathway via targeting autophagy-related gene 12 (Atg12). Furthermore, suppressing miR-188 in the liver of obese mice improved liver steatosis and insulin resistance. Taken together, our findings reveal a new regulatory role of miR-188 in glucose and lipid metabolism through the autophagy pathway, and provide a therapeutic insight for NAFLD.

Supplementary Materials

    • Supplementary Figure 1
    • Table 1. MiR-188 predicted targets.

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Print ISSN:
0022-0795
Online ISSN:
1479-6805
Copyright:
© 2020 Society for Endocrinology 2020
Page(s):
13
Article Type:
Research Article
Received Date:
30 Mar 2020
Accepted Date:
03 Apr 2020
Print Publication Date:
01 Jun 2020
Online Publication Date:
Jun 2020
Keywords:
miR-188; NAFLD; insulin resistance; liver steatosis; autophagy

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