Adult pigeons when given extended treatment with beef anterior pituitary extract will become refractory to the prolactin and gonadotrophic portions of the extract. Refractoriness to prolactin is indicated by the failure of the crops of the immunized birds to continue to respond, with typical hyperplasia, to the prolactin entity of the administered extract. That an antigonadotrophic factor is produced in the immunized adult pigeons is suggested by the finding that the spermatogenic activity in these birds was much less than that noted in birds receiving short-term treatment with anterior pituitary extract. In addition, the serum of the immunized birds significantly reduced the effects of the anterior pituitary extract on average size of ovarian follicles and hypertrophy of the epididymis in immature pigeons.
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