Leptin system loss of function in the absence of obesity in zebrafish

in Journal of Endocrinology
Authors:
Amrutha Bagivalu Lakshminarasimha Institute of Zoology, University of Cologne, Cologne, Germany

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Patrick Page-McCaw Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee, USA

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Diana Möckel Department of Nanomedicine and Theranostics, Institute for Experimental Molecular Imaging, Faculty of Medicine, RWTH Aachen University, Aachen, Germany

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Felix Gremse Department of Nanomedicine and Theranostics, Institute for Experimental Molecular Imaging, Faculty of Medicine, RWTH Aachen University, Aachen, Germany
Gremse-IT GmbH, Aachen, Germany

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Maximilian Michel Institute of Zoology, University of Cologne, Cologne, Germany

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https://orcid.org/0000-0002-5910-5363

Correspondence should be addressed to M Michel: maximichel@gmail.com
DOI:
https://doi.org/10.1530/JOE-21-0037
Volume/Issue:
Volume 251: Issue 1
Article Type:
Research Article
Page Range:
41–52
Online Publication Date:
16 Aug 2021
Copyright:
© Society for Endocrinology 2021
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The leptin system plays a crucial role in the regulation of appetite and energy homeostasis in vertebrates. While the phenotype of morbid obesity due to leptin (Lep) or leptin receptor (LEPR) loss of function is well established in mammals, evidence in fish is controversial, questioning the role of leptin as the vertebrate adipostat. Here we report on three (Lepr) loss of function (LOF) and one leptin loss of function alleles in zebrafish. In order to demonstrate that the Lepr LOF alleles cannot transduce a leptin signal, we measured socs3a transcription after i.p. leptin which is abolished by Lepr LOF. None of the Lepr/Lepa LOF alleles leads to obesity/a body growth phenotype. We explore possible reasons leading to the difference in published results and find that even slight changes in background genetics such as inbreeding siblings and cousins can lead to significant variance in growth.

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Print ISSN:
0022-0795
Online ISSN:
1479-6805
Copyright:
© Society for Endocrinology 2021
Page(s):
12
Article Type:
Research Article
Received Date:
05 Jun 2021
Accepted Date:
30 Jun 2021
Print Publication Date:
01 Oct 2021
Online Publication Date:
16 Aug 2021
Keywords:
leptin; leptin receptor; zebrafish

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