Naltrexone/bupropion modifies weight, food intake, and Drd2 gene expression in rats

in Journal of Endocrinology
Authors:
Gilvanildo Roberto da Silva Department of Pathology, Federal University of Pernambuco, Recife, Brazil
Agrarian Sector, Federal University of Paraná, Veterinary Hospital, Curitiba, Brazil

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Mariane Gomes Carneiro Department of Pathology, Federal University of Pernambuco, Recife, Brazil

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Miriam Pereira Barbosa Department of Biomedicine, Federal University of Pernambuco, Recife, Brazil

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Jaciane de Almeida Costa Department of Pathology, Federal University of Pernambuco, Recife, Brazil

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Ivone Antonia de Souza Department of Pharmacology, Federal University of Pernambuco, Recife, Brazil

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Lisiane dos Santos Oliveira Academic Center of Vitória de Santo Antão, Federal University of Pernambuco, Recife, Brazil

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Diogo Antonio Alves de Vasconcelos Department of Nutrition, Federal University of Pernambuco, Recife, Brazil

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Elizabeth do Nascimento Department of Nutrition, Federal University of Pernambuco, Recife, Brazil

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Rhowena Jane Barbosa Matos Health Sciences Center, Federal University of Reconcavo da Bahia, Cruz das Almas, Brazil

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Sandra Lopes de Souza Department of Anatomy, Federal University of Pernambuco, Recife, Brazil

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Manuela Figueiroa Lyra de Freitas Department of Pathology, Federal University of Pernambuco, Recife, Brazil
Department of Anatomy, Federal University of Pernambuco, Recife, Brazil

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Correspondence should be addressed to G Roberto da Silva: gilvanildoroberto@hotmail.com
DOI:
https://doi.org/10.1530/JOE-21-0393
Volume/Issue:
Volume 253: Issue 3
Article Type:
Research Article
Page Range:
85–96
Online Publication Date:
13 Apr 2022
Copyright:
© Society for Endocrinology 2022
Restricted access
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Obesogenic diets are known to induce obesity and changes in food intake in experimental animals. Obesity negatively affects the peripheral metabolism and neural aspects, such as changes in eating behavior. In obese animals, dopamine (DA) receptor levels are reduced. DA is one of the main peptides involved in the motivation and pleasure of eating. A combination of naltrexone/bupropion (NB) has shown promise in controlling metabolic alterations, but there are few studies on how they modulate dopaminergic expression. NB, in addition to reducing food intake and body weight, can modify tyrosine hydroxylase (Th) and DA receptor D2 (Drd2) levels in the mesolimbic areas of rats submitted to a high-fat diet (HF). The study evaluated the effect of NB on food intake, body weight, and expression levels of Th, Drd1a, and Drd2, in the nucleus accumbens and striatum of rats fed on HF diet. Wistar rats were grouped according to diet: standard (n  = 20) and HF diet (n  = 20). The food intake and body weight were analyzed. The gene expression of Th, Drd1a, and Drd2 was evaluated using real-time PCR. NB combination of 1 mg/kg and 20 mg/kg reduced food intake and body weight, increased Drd2 expression in rats on HF diet, and increased Th in rats on both experimental diets. The level of Drd1a was unchanged. We concluded that bodyweight reduction may be associated with decreased food intake in response to the increased Drd2 expression in the mesolimbic areas of rats that received an HF diet.

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Print ISSN:
0022-0795
Online ISSN:
1479-6805
Copyright:
© Society for Endocrinology 2022
Page(s):
12
Article Type:
Research Article
Received Date:
25 Nov 2021
Accepted Date:
18 Mar 2022
Print Publication Date:
01 Jun 2022
Online Publication Date:
13 Apr 2022
Keywords:
bupropion; dopamine receptors; naltrexone; obesity; mRNA expression; rats

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