Leptin modulates oocyte maturation by a central and a direct pathway in zebrafish

in Journal of Endocrinology
Authors:
Amrutha Bagivalu Lakshminarasimha Institute of Zoology, University of Cologne, Cologne, Germany

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Madhuri Puvvada Institute of Zoology, University of Cologne, Cologne, Germany

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Matthias Hammerschmidt Institute of Zoology, University of Cologne, Cologne, Germany

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Maximilian Michel Institute of Zoology, University of Cologne, Cologne, Germany

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https://orcid.org/0000-0002-5910-5363

Correspondence should be addressed to M Michel: maximichel@gmail.com
DOI:
https://doi.org/10.1530/JOE-22-0026
Volume/Issue:
Volume 254: Issue 1
Article Type:
Research Article
Page Range:
1–12
Online Publication Date:
10 May 2022
Copyright:
© Society for Endocrinology 2022
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Loss of LEPR function (LOF) in mammals leads to diverse phenotypes including morbid obesity and infertility while zebrafish show relatively minor phenotypes. This however allows the study of LEPR LOF in the absence of the detrimental effects of hyperglycemia or obesity. Here, we show evidence that leptin plays a role in the central as well as peripheral regulation of the hypothalamic–pituitary–gonadal (HPG) axis in zebrafish. Animals with a Lepr LOF show dysregulated pituitary HPG genes as well as evidence that oocytes mature slower and/or exhibit an increased rate of atresia. In culture, Lepr LOF attenuates the effect of 17α-20β-dihydroxy-4 pregnen-3-one in promoting germinal vesicle breakdown (GVBD) and increases the rate of GVBD as well as attenuates the rate of oocyte atresia.

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Print ISSN:
0022-0795
Online ISSN:
1479-6805
Copyright:
© Society for Endocrinology 2022
Page(s):
12
Article Type:
Research Article
Received Date:
21 Mar 2022
Accepted Date:
05 Apr 2022
Print Publication Date:
01 Jul 2022
Online Publication Date:
10 May 2022
Keywords:
LepR; leptin; oocyte; GVBD; zebrafish

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