Quercetin improves white adipose tissue redox homeostasis in ovariectomized rats

in Journal of Endocrinology
Authors:
Leonardo Matta Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
Institute for Cardiovascular Prevention, Ludwig-Maximilians-University Munich, Munich, Germany
Institute for Diabetes and Cancer, Helmholtz Center Munich, Munich, Germany

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Cinthia Breves Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

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Luiz Fonte Boa Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

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Aina Eiras Domingos Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

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Caroline C Faria Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
UMR9019 CNRS, Université Paris-Saclay, Institut Gustave Roussy, Villejuif, France

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Itanna Souza Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

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Niedson Correia Lima-Junior Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

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Anna Paola Trindade Rocha Josué de Castro Institute of Nutrition, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

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Bianca M Gregório Urogenital Research Unit, State University of Rio de Janeiro, Rio de Janeiro, Brazil

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Denise Pires Carvalho Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

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Andrea C F Ferreira Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
Josué de Castro Institute of Nutrition, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

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José Hamilton Matheus Nascimento Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

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Leonardo Maciel NUMPEX, Duque de Caxias Campus, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

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Rodrigo S Fortunato Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

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Correspondence should be addressed to R S Fortunato: rodrigof@biof.ufrj.br

*(L Matta and C Breves contributed equally to this work)

DOI:
https://doi.org/10.1530/JOE-23-0166
Volume/Issue:
Volume 259: Issue 2
Article Type:
Research Article
Article ID:
e230166
Online Publication Date:
25 Sep 2023
Copyright:
© the author(s) 2023
Restricted access
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Estrogen deficiency is a well-known hallmark of menopause and is associated with oxidative stress and metabolic dysfunction. Quercetin (Q), a flavonoid found in fruits and vegetables, has demonstrated anti-inflammatory effects in experimental models of metabolic disorders. In this study, we aimed to investigate the effects of quercetin on retroperitoneal white adipose tissue (rWAT) redox homeostasis and systemic metabolic parameters in ovariectomized (OVX) rats. Female Wistar rats at 3 months old were divided into the following experimental groups: sham-operated treated with vehicle (DMSO 10% + PBS – 1 mL/kg); OVX (vehicle treated) and OVX-Q (25 mg/kg) – via oral gavage, daily for 5 weeks. Q did not prevent weight gain but improved glucose tolerance and blood cholesterol profile, and attenuated uterine atrophy in OVX rats. Furthermore, Q had a protective effect on rWAT, once the OVX-Q group presented lower oxidative stress levels, and reduced levels of the pro-inflammatory cytokine tumor necrosis factor alpha, compared to the OVX group. Q improved antioxidant enzyme activities such as superoxide dismutase and catalase and decreased reactive oxygen species production, in OVX-Q rats. It was followed by increased levels of total thiol content and lower lipid peroxidation. Moreover, Q reduced senescent-related genes p16INK4a and p19ARF expression which were higher in the OVX group. In conclusion, quercetin supplementation improved redox homeostasis and reduced senescence-related markers, and inflammation in rWAT, which was reflected in preserved systemic metabolic health parameters in OVX rats. These findings suggest that quercetin may have therapeutic potential for the management of metabolic disorders associated with menopause-induced estrogen deficiency.

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Print ISSN:
0022-0795
Online ISSN:
1479-6805
Copyright:
© the author(s) 2023
Article ID:
e230166
Article Type:
Research Article
Received Date:
25 May 2023
Accepted Date:
11 Aug 2023
Print Publication Date:
01 Nov 2023
Online Publication Date:
25 Sep 2023
Keywords:
adipose tissue; metabolic dysfunction; oxidative stress; ovariectomy

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