There is considerable evidence that β-glucuronidase (β-G) activity, especially in the reproduction tract, is concentrated at sites where oestrogenic and androgenic hormones exert their actions. Biochemical assays (Fishman, 1955) have demonstrated a high level of β-G activity in mammalian ovaries, and the present work is a brief account of the distribution of this enzyme in the ovaries of the rat and rabbit. The ovaries were fixed in cold chloral formalin immediately after the death of the animal and frozen sections were prepared using the modified ferric hydroxyquinoline technique described by Fishman & Baker (1956).
The findings reported by these workers with the original description of their method can be elaborated and somewhat modified. In the ovary of the non-pregnant rat β-G activity is concentrated mainly in the germinal epithelium, in the granulosa cells of young follicles, in the outer layers of the granulosa of older follicles and in the muscular
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