Microbiome dysbiosis by antibiotics protects cartilage degradation in OAOP mice

in Journal of Endocrinology
Authors:
Qin Yin Department of Orthopedics, Wuxi Ninth People’s Hospital Affiliated to Soochow University, Wuxi, Jiangsu, China

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Jun Gu Department of Orthopedics, Wuxi Ninth People’s Hospital Affiliated to Soochow University, Wuxi, Jiangsu, China

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Pengju Ren Department of Orthopedics, The Shanghai Tenth People’s Hospital of Tongji University, Shanghai, China

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Zhiqiang Guan Department of Dermatology, Xuzhou Municipal Hospital Affiliated to Xuzhou Medical University, Xuzhou, Jiangsu, China

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https://orcid.org/0000-0002-6053-2717
,
Yongxiang Wang Department of Orthopedics, Clinical Medical College, Yangzhou University, Yangzhou, China
Department of Orthopedics, Northern Jiangsu People’s Hospital, Yangzhou, China

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Ruijun Bai Department of Orthopedics, Wuxi Ninth People’s Hospital Affiliated to Soochow University, Wuxi, Jiangsu, China

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Yu Liu Department of Orthopedics, Wuxi Ninth People’s Hospital Affiliated to Soochow University, Wuxi, Jiangsu, China

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Correspondence should be addressed to J Gu or Y Liu: arielle2008@163.com or liuyu2956@suda.edu.cn

*(Q Yin, P Ren and Z Guan contributed equally to this work)

DOI:
https://doi.org/10.1530/JOE-23-0330
Volume/Issue:
Volume 261: Issue 2
Article Type:
Research Article
Article ID:
e230330
Online Publication Date:
13 Mar 2024
Copyright:
© the author(s) 2024
Restricted access
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The role of this study was to evaluate the impact of gut microbiota depletion on the progression of osteoarthritis (OA) and osteoporosis (OP). We conducted an experimental mouse model of OA and OP over an 8-week period. The model involved destabilization of the medial meniscus and bilateral ovariectomy (OVX). To deplete the gut microbiota, we administered a course of antibiotics for 8 weeks. The severity of OA was assessed through micro-CT scanning, X-rays, and immunohistochemical staining. Microbiome analysis was performed using PCR of 16S DNA on fecal samples, and the levels of serum lipopolysaccharide, interleukin 6, tumor necrosis factor-α (TNF-α), osteocalcin, and estrogen were measured using enzyme-linked immunosorbent assay. We found that in comparison to the OVX+OA group, the OVX+OA+ABT group exhibited increased bone mineral density (P < 0.0001), bone volume fraction (P = 0.0051), and trabecular number (P = 0.0023) in the metaphyseal bone. Additionally, cartilage injury and levels of matrix metalloproteinase 13 were reduced in the OVX+OA+ABT group compared to the OVX+OA group. Moreover, the OVX+OA+ABT group demonstrated decreased relative abundance of Bacteroidetes, serum lipopolysaccharide (P = 0.0005), TNF-α (P < 0.0001), CTX-1 (P = 0.0002), and increased expression of bone formation markers. These findings were further supported by correlation network analyses. Depletion of gut microbiota was shown to protect against bone loss and cartilage degradation by modulating the composition of the gut microbiota in osteoporosis and osteoarthritis.

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Print ISSN:
0022-0795
Online ISSN:
1479-6805
Copyright:
© the author(s) 2024
Article ID:
e230330
Article Type:
Research Article
Received Date:
24 Oct 2023
Accepted Date:
24 Jan 2024
Print Publication Date:
01 May 2024
Online Publication Date:
13 Mar 2024
Keywords:
gut microbiota; osteoarthritis; osteoporosis; LPS

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