Absence of sex differences in diabetes-induced suppression of KNDy neurons in rats

in Journal of Endocrinology
Authors:
Mai Otsuka Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School, Bunkyo-ku, Tokyo, Japan

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Kinuyo Iwata Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School, Bunkyo-ku, Tokyo, Japan

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Yuki Otsuka Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya, Aichi , Japan

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Yoshihisa Uenoyama Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya, Aichi , Japan

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Hitoshi Ozawa Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School, Bunkyo-ku, Tokyo, Japan
Faculty of Health Sciences, Bukkyo University, Nakagyo-ku, Kyoto, Japan

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Hirotaka Ishii Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School, Bunkyo-ku, Tokyo, Japan

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Correspondence should be addressed to K Iwata: kiwata0309@nms.ac.jp
DOI:
https://doi.org/10.1530/JOE-23-0293
Volume/Issue:
Volume 263: Issue 3
Article Type:
Research Article
Article ID:
e230293
Online Publication Date:
20 Nov 2024
Copyright:
© the author(s) 2024
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Diabetes mellitus disturbs kisspeptin–neurokinin B–dynorphin A (KNDy) neurons in the arcuate nucleus (ARC), which regulate pulsatile luteinizing hormone (LH) secretion in both sexes. However, it remains unclear whether a sex-specific association with the negative effects of diabetes on KNDy neurons exists. Therefore, we examined mRNA expression in KNDy neurons of diabetic male and female rats 7 weeks after streptozotocin (STZ) injection using histochemistry. In gonad-intact rats, the numbers of Kiss1 and Pdyn mRNA-expressing cells in the ARC decreased in an STZ-dose-dependent manner; moreover, no sex-dependent association with STZ treatment was observed. In males, plasma LH and sex steroid levels decreased in diabetic rats. Conversely, those of females did not vary significantly between the control and diabetic rats. Kiss1 expression in the anteroventral periventricular nucleus was slightly affected in diabetic animals but did not exhibit sex-dependent differences. In gonadectomized rats, the numbers of KNDy mRNA-expressing cells in the ARC and plasma LH levels decreased in diabetic male and female rats; however, sex-dependent differences did not exist. These results demonstrated that a sex-specific association with the negative effects of diabetes on KNDy neurons did not exist. Therefore, the stage of diabetes that induces the suppression of the hypothalamus may not vary according to sex.

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Print ISSN:
0022-0795
Online ISSN:
1479-6805
Copyright:
© the author(s) 2024
Article ID:
e230293
Article Type:
Research Article
Received Date:
14 Sep 2023
Accepted Date:
08 Oct 2024
Print Publication Date:
01 Dec 2024
Online Publication Date:
20 Nov 2024
Keywords:
diabetes mellitus; estrogen; kisspeptin–neurokinin B–dynorphin neurons; luteinizing hormone; testosterone

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