The nitrogen distribution of uterine washings differs from that of blood plasma (Ringler, 1956; Junge & Blandau, 1958; Heap & Lamming, 1962). The acid-soluble nitrogen, soluble in an equal volume of 11% trichloracetic acid, represents a considerable proportion of the total nitrogen of the uterine washing in the rat at oestrus, in the rabbit during the luteal phase and in the cow during the latter half of the luteal phase but not to the same extent in the sheep (Heap, 1962; Heap & Lamming, 1962). The occurrence of the acid-soluble component was associated with a translucent appearance of the trichloracetic acid (TCA) extract of uterine washings. It was also extracted with lipid solvents. The levels of acid-soluble nitrogen and of total carbohydrate in uterine washings of the rat and rabbit showed a positive correlation (Heap, 1962; Heap & Lamming, 1962).
Seromucoid in the TCA extract of uterine washings was precipitated
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