Measurement of minute amounts of adrenocorticotrophic hormone (ACTH) in body fluids at present depends upon biological assay. We here report a very sensitive method of ACTH assay using the transplanted gland in sheep whose ACTH secretions have been suppressed with 16α-methyl-9α-fluoro-Δ1 cortisol (dexamethasone).
The animals used were three Merino ewes. The left adrenal had been transplanted to a vascular anastomosis with the left carotid and jugular vessels and enclosed in a skin loop 4–8 months previously, using the technique of McDonald, Goding & Wright (1958). The right adrenal was removed 2–4 weeks later. Experiments were conducted on each animal in turn at monthly intervals, when blood was collected from the adreno-jugular vein before and after carotid-arterial injections of test fluid. A fluorescent technique (De Moor, Raskin & Steeno, 1960) was used to measure cortisol—the main glucocorticoid secreted by the sheep (Bush & Ferguson, 1953)—in 2 ml. samples of adrenal
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