This communication presents a technique for induction of deciduomata in the pseudopregnant hamster which is simple, reliable, and relatively non-traumatic; the decidualization resembles in pattern and distribution that of normal pregnancy. Such deciduomata may be induced by injection of air during the 4th day (i.e. 3 days and 9–22 hr. after ovulation) of pseudopregnancy induced by mating with a vasectomized male.
The ovarian end of each uterus, exposed through a dorsolateral incision, is held between the fingers and the needle inserted into the ovarian end of the lumen. With a tuberculin syringe and a 26 gauge, ⅜ in. needle, 0·05 ml. of air is injected into the uterine lumen; a drop of Vaseline placed about the upper end of the plunger prevents slipping and allows exact control of the volume. Although the uterus is slightly distended by the injections, little bleeding occurs and no internal sutures are required. Autopsy at
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Online ISSN: 1479-6805
Print ISSN: 0022-0795
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