The possibility of applying the ovarian ascorbic acid depletion assay or its variants to the measurement of luteinizing hormone in human urine extracts has been investigated.
A 3 hr. i.v. technique proved the most sensitive.
The method had limitations as a clinical assay, namely: a relatively low degree of precision, toxicity of some urinary extracts and the fact that about 20 % of assays were invalid owing to a lack of parallelism.
Two of ten patients with amenorrhoea and raised gonadotrophin levels showed a relative lack of FSH as compared with the International Reference Preparation for human menopausal gonadotrophin.
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Online ISSN: 1479-6805
Print ISSN: 0022-0795
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