Green & Matty (1963) reported that both metabolic rate and active sodium transport increased within minutes after the addition of thyroxine (T4) to solutions bathing the serosal surface of ventral skin and urinary bladders from the toad, Bufo bufo. The response persisted at least 1 hr. with peak increases in O2 consumption averaging well over 100% for both skin and bladder when determined polarographically. More recently, using standard Warburg manometry, Thornburn & Matty (1964) obtained only about 25% increase in O2 consumption from toad bladders treated with T4 and no response from toad skin. However, skin did respond to triiodothyronine and tetraiodothyroacetic acid with increases of 13 and 8%, respectively. Active sodium transport, measured as short-circuit current, increased about 100 % in bladder and 50 % in skin after exposure of the serosal surface to T4 at a concentration of 10−6 m (Green & Matty, 1963).
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