Both heating (Laron, Yed-Lekach, Assa & Kowadlo-Silbergeld, 1964a) and enzyme digestion (Laron, Yed-Lekach, Assa & Kowadlo-Silbergeld, 1964b) alter the immunological properties of human and bovine growth hormone. The purpose of the present investigation was to study the effect of heat and peptic digestion on the immunological properties of sheep prolactin, which has an electrophoretic mobility similar to that of human growth hormone (Laron & Assa, 1963).
Sheep prolactin was supplied by the Endocrinological Study Section of the U.S. National Institute of Health; batches P-S-3 and P-S-4 were used. The preparation of antiserum and the procedures of agar gel double diffusion, electrophoresis and immunoelectrophoresis were performed as previously described (Laron & Assa, 1962). Test tubes containing 2 mg. hormone dissolved in 0·2 ml. water were heated in a water bath at 100° at pH 5·5–6·5. The electrophoretic and immunological properties of the heated hormone were tested at intervals of
Journal of Endocrinology is committed to supporting researchers in demonstrating the impact of their articles published in the journal.
The two types of article metrics we measure are (i) more traditional full-text views and pdf downloads, and (ii) Altmetric data, which shows the wider impact of articles in a range of non-traditional sources, such as social media.
More information is on the Reasons to publish page.
Sept 2018 onwards | Past Year | Past 30 Days | |
---|---|---|---|
Full Text Views | 1 | 0 | 0 |
PDF Downloads | 2 | 0 | 0 |