The NIH-B2-GH preparation of ox growth hormone (GH) was separated by chromatography on DEAE-cellulose into six fractions. Five of these fractions when assayed in hypophysectomized rats showed GH activity ranging in potency from 0·25 to 2·5 times the starting material. Growth activity could not be correlated with the concentration of any single component revealed by starch gel electrophoresis.
Antisera produced to NIH-B2-GH had antihormone activity and produced two precipitin lines in Ouchterlony diffusion tests. One of these lines was associated with serum γ-globulin and was shared by all five fractions. The other line was present in only two of the fractions, and these contained the more anionic components.
It is suggested that the more cationic growth-active components present in bovine and ovine GH preparations do not readily produce precipitating antibodies and that this may complicate the results of precipitin and gel diffusion tests when heterogeneous GH preparations have been used to prepare the antisera.
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