(1) Two methods for the preparation of radioactive iodine-labelled insulin of high specific activity are described.
(2) Gel filtration on Sephadex proved to be a suitable method for purification of the labelled insulin.
(3) No loss of antigenicity of the labelled hormone could be detected.
(4) Bioassays of the labelled insulin showed that it was as active as the non-labelled hormone.
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