The routine procedure in this laboratory for the extraction of gonadotrophins from urine is a kaolin-acetone method (Brown, 1959). Recently, we have started using a new bioassay depending on induced ovulation in mice (Cunningham, 1962). The present investigation was undertaken to discover whether any other extraction methods would give consistently higher yields than Brown's method when gonadotrophin was measured by the ovulation assay. The methods selected are shown in Table 1. They were chosen as being comparable in speed and convenience with the method in present use; more laborious techniques such as those employing ethanol precipitation were excluded since they are unsuitable for routine use.
Techniques of extraction and grades of reagents were all similar to those specified in the published descriptions. Where the specified quantities were given in relation to a 24 hr. specimen, this was taken as equivalent to 1200–1500 ml. urine. The dried extracts were stored at
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