A BIOLOGICAL ASSAY FOR CALCITONIN

in Journal of Endocrinology
Authors:
M. A. KUMAR
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E. SLACK
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A. EDWARDS
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H. A. SOLIMAN
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A. BAGHDIANTZ
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G. V. FOSTER
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I. MacINTYRE
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SUMMARY

(1) Calcitonin preparations from acetone-dried thyroid were administered to rats by various routes.

(2) Intravenous administration, especially by infusion, produced a much greater fall in plasma calcium than s.c. or i.p. injection.

(3) The log dose-effect curves after i.v. injection or infusion showed no evidence of non-linearity over a 100-fold dose range and had highly significant slopes.

(4) The potency ratio of two preparations was estimated by means of a (2+2) assay design using both i.v. infusion and single i.v. injection. There was satisfactory agreement.

(5) The i.v. injection method is recommended for the routine assay of calcitonin. A simple assay schedule is given in the Appendix.

(6) A unit of calcitonin activity is defined in terms of a standard preparation.

 

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