Bell, Mukerji & Loraine (1964) have described a bioassay method for the quantitative determination of luteinizing hormone (LH) depending on ovarian cholesterol depletion (OCD test) after i.p. administration of the hormone. The assay is conducted in intact, immature rats pretreated with 50 i.u. pregnant mare serum gonadotrophin (PMSG) followed 72 hr. later by 25 i.u. human chorionic gonadotrophin (HCG). As described by Bell et al. (1964) the bioassay proper is performed on the 11th day after the administration of HCG at which time the ovarian cholesterol level generally varies from 1000 to 2000μg./100 mg. ovary (Bell & Mukerji, unpublished observations). The main practical difficulty associated with the method is the lack of uniformity of the ovaries in any given assay both in relation to their appearance and their cholesterol content. This difficulty was further emphasized by Mukerji, Bell & Loraine (1965) who showed that the concentration of cholesterol varied considerably
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