Isolated tissues of the toad Bufo bufo (urinary bladder, skin and kidney) were treated in vitro with thyroxine (T4) or tri-iodothyronine (T3) both with and without prior administration of the hormones in vivo. They were incubated at low temperature, and their oxygen consumption studied for 48 hr. Both hormones had little effect in vitro. Pretreatment with T4 in vivo usually lowered oxygen uptake; the effect of pretreatment with T3 varied. The presence of alanine in the incubating medium caused a marked increase in oxygen consumption. A seasonal effect was also found.
The respiration of isolated toad bladder was stimulated by a wide range of concentrations of thyroxine in vitro, and there were two peaks of stimulation.
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