After the injection of cortisol into the toad Xenopus laevis, the concentration of steroid in the main circulatory system was much higher than that in the limb muscles. The concentration of corticosteroids in the blood fell at a faster rate than that in muscle. The particulate fraction of toad muscle homogenate bound added cortisol and some of it was not removed by repeated washing of the tissue with fresh medium. Bound steroid was not confined to any one subcellular fraction. The small particle fraction ('microsomes') contained the greatest proportion of steroid and the highest steroid concentration. The loss of corticosteroids from intact and washed particle preparations of toad gastrocnemius was not influenced by temperature over the range of 17–37°.
Copper, zinc and manganese (between 26 and 31 mm) inhibited the release of cortisol bound to muscle particles; p-chloromercuribenzoate (2 mm) and anoxia had no effect. A variation in pH from 2 to 10 made little difference to the rate of steroid release by muscle particles suspended in 0·15 m-KC1. The results suggest that the release of strongly bound steroids from muscle does not involve an enzymic mechanism.
Journal of Endocrinology is committed to supporting researchers in demonstrating the impact of their articles published in the journal.
The two types of article metrics we measure are (i) more traditional full-text views and pdf downloads, and (ii) Altmetric data, which shows the wider impact of articles in a range of non-traditional sources, such as social media.
More information is on the Reasons to publish page.
| Sept 2018 onwards | Past Year | Past 30 Days | |
|---|---|---|---|
| Full Text Views | 2 | 0 | 0 |
| PDF Downloads | 1 | 0 | 0 |
Online ISSN: 1479-6805
Print ISSN: 0022-0795
Strengthening biomedical communities
to advance science and health
CONTACT US
Bioscientifica Ltd | Starling House | 1600 Bristol Parkway North | Bristol BS34 8YU | UK
Bioscientifica Ltd | Registered in England no 3190519
