As much as 70 % of the urinary metabolites of labelled progesterone in the rabbit consist of compounds which can be extracted from ether into aqueous sodium bicarbonate (Cooke, Rogers & Thomas, 1963). In view of the quantitative significance of this group of metabolites it was of interest to establish whether androstenedione also gave rise to bicarbonate-extractable metabolites.
Four mature Dutch female rabbits were injected subcutaneously with 0·15, 15, 25 and 50 mg. of [4-14C]androstenedione (8 μc) respectively, and urine was collected for 65 hr. The following procedures (designated as A, B and C in Fig. 1) were used for the hydrolysis of the steroid conjugates:
In two experiments (when 0·15 and 15 mg. of steroid were injected) 15% (v/v) concentrated hydrochloric acid was added to the urine, and the solution was heated on a boiling water bath for 30 min.
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