Pooled urine from various groups of human subjects was extracted by the kaolin-acetone procedure of Albert (A procedure) and that of Loraine & Brown (L-B procedure). Two to three times as much solid was obtained with the L-B procedure. When the biological activity of the extracts was compared by the mouse uterine weight assay, they had similar potencies when the assay was conducted with small doses of extract. When larger doses of extract were used the A extract was 2·25 times as active as the L-B extract. Extracts obtained by both procedures contained gonadotrophin inhibiting material, but the L-B extract was 3·5 times more active in this respect. It was concluded that this was the reason for the apparent difference in response to the extracts at the higher dosage.
Gel filtration studies indicated that NaOH elution from kaolin and precipitation with 5 vol. acetone (as used in the L-B procedure), extracted more gonadotrophin-inhibiting material. Since this material was without gonadotrophic activity, NH4OH elution with 2 vol. acetone precipitation (as used in the A procedure) is preferable for urine extraction before gonadotrophin assays.
Journal of Endocrinology is committed to supporting researchers in demonstrating the impact of their articles published in the journal.
The two types of article metrics we measure are (i) more traditional full-text views and pdf downloads, and (ii) Altmetric data, which shows the wider impact of articles in a range of non-traditional sources, such as social media.
More information is on the Reasons to publish page.
| Sept 2018 onwards | Past Year | Past 30 Days | |
|---|---|---|---|
| Full Text Views | 0 | 0 | 0 |
| PDF Downloads | 2 | 1 | 0 |
Online ISSN: 1479-6805
Print ISSN: 0022-0795
Strengthening biomedical communities
to advance science and health
CONTACT US
Bioscientifica Ltd | Starling House | 1600 Bristol Parkway North | Bristol BS34 8YU | UK
Bioscientifica Ltd | Registered in England no 3190519
